Melatonin improves the first cleavage of parthenogenetic embryos from vitrified–warmed mouse oocytes potentially by promoting cell cycle progression

Table 1 Information of primers used in RT-qPCR

Genes	Accession ID	Primer Seq (5′→3′)	Product Length, bp	Tm, °C
Btg4	NM_019493.4	F: TGAACAACCCAAAGAGCGTCTACC	103	55
Btg4	NM_019493.4	R: AACCCACGACCATCTGCCAAATG	103	55
Orc2	NM_001025378.2	F: TGATTCATGTCTTACGAAGCCT	107	55
Orc2	NM_001025378.2	R: AGAAAGTCCAATGTAGGAAGGG	107	55
Eif4e	NM_001313980.1	F: ACTTTTGGGCTCTATACAACCA	82	55
Eif4e	NM_001313980.1	R: ATCCCGTCCTTAAAAAGTGAGT	82	55
Miss	NM_001045483.1	F: GTCCTTTAGGTACACAGGGATC	207	55
Miss	NM_001045483.1	R: GATATGACGCTTCAGGAGTAGG	207	55
Doc1r	NM_026373.4	F:ACGGACCTGCTGTCTGTCATAGAG	146	55
Doc1r	NM_026373.4	R: TTGCGTTCTGTCTCTGCCAAGC	146	55
Ythdf2	NM_145393.4	F:TTGCCTCCACCTCCACCACAG	111	55
Ythdf2	NM_145393.4	R:CCCATTATGACCGAACCCACTGC	111	55
Setd2	NM_001081340.2	F: GGAGGCAGACACGGAGACAGAG	139	55
Setd2	NM_001081340.2	R: ATCTGGTGGCTCCTGGCTTCTC	139	55
Gapdh	NM_008084.3	F: CATGGCCTTCCGTGTTCCTA	104	55
Gapdh	NM_008084.3	R: GCCTGCTTACCACCTTCTT	104	55

ISSN: 2049-1891